Abstract:

The relevance of the effective and the most accurate diagnosis of dengue is essential for the proper analysis of clinical sample, its surveillance activity, outbreak control, pathogenesis, academic research, and other trails. Several clinical tests can be performed to identify and detect the viral load, the antigen-antibody reaction, and viral nucleic acid. Nucleic acid detection is the most sensitive and specific method of Dengue diagnosis. Viral nucleic acid appears earlier than antibodies so the infection can be detected at very early stage. This is an advance method by which further research such as molecular characterization of infecting virus can also be done. However, this method is expensive, needs technical expertise and advanced laboratory set up. Several other nucleic acids amplification-based techniques have also been developed to detect Dengue infection such as Loop mediated amplification methods and NASBA. Nucleic Acid Sequence Based Amplification (NASBA) does not require thermo-cycler. After the cDNA synthesis the newly formed double stranded DNA serves as a template for RNA transcription. The amplified RNA is detected by electrochemiluminescence or in real-time with fluorescent-labelled molecular beacon probes

Description:

nucleic acid, serotyping of Dengue virus, antigen-antibody reaction